================================================================
Electronic PCR (e-PCR) program for finding STSs in DNA sequences
================================================================

REFERENCE: G.D.Schuler. Sequence mapping by "Electronic PRC".
           (submitted 23-Dec-96)

FEEDBACK:  schuler@ncbi.nlm.nih.gov

USAGE:  e-PCR <stsfile> <seqfile>

        Where <stsfile> is one of the files in the db directory or
        one that you supply (see below for format specification) 
        and <seqfile> is a FASTA-style file containing any number 
        of sequences.


SYSTEMS SUPPORTED
=================
SGI/UNIX
Sun SPARC/Solaris
Intel PC/Win32 (Win95 or WinNT)


INSTALLATION
============
Just identify the correct executable for your system and copy to a
location that is on the search path.  You can use any of the following 
commands to test the program; they should all produce some output.

    e-PCR db/genethon.sts samples/L78833.nt

    e-PCR db/genethon.sts samples/U47924.nt

    e-PCR db/txmap.sts samples/U47924.nt


STS DATABASE FILES
==================
genethon.sts     Genethon genetic markers (Dib et al, 1996)
txmap.sts        Transcript Map markers (Schuler et al, 1996)


STS DATABASE FILE SPECIFICATION
===============================
The stsfile should contain at least four (optionally five) 
tab-delimited columns:

col-1:  STS name
col-2:  left PCR primer
col-3:  right PCR primer
col-4:  PCR product size
col-5:  (optional) additional text to be included in the output


PACKING LIST
============

./README

./bin/sgi/e-PCR
./bin/solaris/e-PCR
./bin/win32/e-PCR.exe

./db/genethon.sts   
./db/txmap.sts      

./samples/L78833.nt  
./samples/U47924.nt

./src/
./src/e-PCR.cxx
./src/fasta-io.cxx
./src/fasta-io.h
./src/makefile
./src/stsmatch.cxx
./src/stsmatch.h
./src/util.cxx
./src/util.h